Real-time PCR in Practice

For novices as well as experts, this handy benchtop reference covers basic protocols and theoretical background for first-time users, while also addressing advanced methods and strategies for high performance applications. As such, it teaches the basic requirements, practical know-how as well as the hurdles and pitfalls in obtaining meaningful results in readily comprehensible terms. The authors explain the biochemical principles behind this method and discuss examples of its most common applications including an abundance of practical tips and tricks, plus a special troubleshooting section.