Post-translational Processing

This text and its companion volume "Protein Expression: A Practical Approach" form the final part of the PAS mini-series on protein synthesis and processing. This volume begins with a chapter on protein sequencing followed by a chapter on protein folding and import into organelles. The next three chapters cover the three major forms of covalent modification: phosphorylation, glycosylation, and lipid modification. Proteolytic processing is the next topic and the final two chapters are concerned with protein turnover in mammalian cells and yeast. This book is a comprehensive volume of late-1990s methodology and is designed to be used at the bench or away from the bench to gain insight into future experimental approaches. This book is intended for any biomedical researcher wanting to investigate protein expression in cell free systems, viruses, prokaryotic cells, and eukaryotic cells.