G-pcr

G-protein-coupled receptors (GPCRs) are the largest protein family in the human genome and the single biggest target for therapeutic agents. The range of techniques for working on GPCRs is vast and it can be difficult to select the best approach to take. This book focuses on methods that are fundamental to GPCR research and those that will be particularly useful tools in future work. "GPCR: Methods Express" covers radioligand binding (including detection of allosteric modulators); assays of second messengers activated by GPCRs; GTPA A-S binding to monitor receptor activation; quantitative GPCR imaging; recombinant GPCRs; BRET analysis of GPCR complexes; intra-molecular FRET studies of receptor conformation; engineered cysteines for introducing intramolecular disulphide bonds and cysteine scanning accessibility mutagenesis; fluorescent correlation spectroscopy (FCS) for studying GPCR diffusion; identification and proteomic analysis of GPCR phosphorylation; visualisation of GPCR trafficking by ELISA and immunofluorescence; homology modelling.
Each chapter considers the various approaches available and then provides recommended protocols - proven by the authors - with hints and tips for success. This book is an essential laboratory manual for all researchers engaged in GPCR research.